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human calabrese crispr activation pooled library set a11  (Addgene inc)


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    Addgene inc human calabrese crispr activation pooled library set a11
    Human Calabrese Crispr Activation Pooled Library Set A11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+crispr+activation+pooled+library+set+a/Human+CRISPR+Activation+Pooled+Library+(Calabrese+VP64)(Pooled+Library+%231000000110%2C+%2392377%2C+%2392378)/pm40877321-241-1-18
    Average 93 stars, based on 11 article reviews
    human calabrese crispr activation pooled library set a11 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    CRISPR:

    Article Title: Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional programs
    Article Snippet: Single guide RNA (sgRNA) sequences for non-targeting control (NTC) and ACE2 were taken from the Weissman Human Genome-wide CRISPRa-v2 library (Addgene #83978). .. LRRC15 sgRNA sequences and additional ACE2 sgRNA sequences were taken from the Human CRISPR activation pooled library set A (Addgene #92379). .. Sense and antisense strands for each sequence were ordered as DNA oligonucleotides (IDT) with 5′ overhangs of 5′-CACC-3′ on the sense strand oligonucleotide and 5′-AAAC-3′ on the antisense strand oligonucleotide.

    Article Title: LRP8‐mediated selenocysteine uptake is a targetable vulnerability in MYCN‐amplified neuroblastoma
    Article Snippet: Following antibodies were used against β‐actin (1:5,000; A5441, Sigma‐Aldrich), GPX4 (1:1,000; no. ab125066, abcam), LRP8 (1:1,000; ab108208, abcam), SLC3A2 (CD98, 1:1,000; sc‐376815, Santa Cruz), SLC7A11 (1:10; antibody raised against a N‐terminal peptide of SLC7A11, clone 15C2‐111, developed in Munich + NaN 3 ), SCLY (1:50; IG‐P1097, ImmunoGlobe GmbH), Flag (1:3,000; F3165, Sigma‐Aldrich), GPX1 (1:1,000; #3286, Cell Signaling), mouse Slc7a11 (1:10; monoclonal antibody generated at the HMGU, clone 4B3‐1113, developed in Munich + NaN 3 ). .. The human CRISPR activation pooled library Set A (Addgene plasmid #92379 was a gift from David Root and John Doench) was amplified as described previously (Joung et al , ). .. Briefly, Endura electrocompetent cells (Lucigen, Cat. No. 60242) were used to perform six electroporation reactions on a MicroPulserTM II (BioRad) following the manufacturer's instructions (pre‐set EC1 setting with V: 1.8 kV).

    Article Title: Selenocysteine metabolism is a targetable vulnerability in MYCN -amplified cancers
    Article Snippet: Cell lines were tested for their identity by SNP genotyping and for mycoplasma contamination by the respective Multiplexion service (Heidelberg, Germany). .. The human CRISPR activation pooled library Set A (Addgene plasmid #92379 was a gift from David Root and John Doench) was amplified as described previously ( ). .. Briefly, Endura electrocompetent cells (Lucigen, Cat. No. 60242) were used to perform six electroporation reactions on a MicroPulserTM II (BioRad) following the manufacturer’s instructions (pre-set EC1 setting with V: 1.8 kV).

    Article Title: Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional programs.
    Article Snippet: .. MegaX DH10B T1R Electrocomp Cells (Thermo Fisher Scientific) were electroporated with 400 ng Human CRISPR activation pooled library set A (Addgene #92379) and left to recover in Recovery Medium for 1 h at 37 ̊C. .. Cells were then spread on 600 cm2 LB-agar plates supplemented with carbenicillin (Merck) and incubated at 37 ̊C for 16 h. All colonies were scraped, collected, and processed using the PureYield Plasmid Maxiprep System (Promega Corporation).

    Article Title: LRP8-mediated selenocysteine uptake is a targetable vulnerability in MYCN-amplified neuroblastoma.
    Article Snippet: Ferroptosis has emerged as an attractive strategy in cancer therapy.. Understanding the operational networks regulating ferroptosis may unravel vulnerabilities that could be harnessed for therapeutic benefit.. Using CRISPR-activation screens in ferroptosis hypersensitive cells, we identify the selenoprotein P (SELENOP) receptor, LRP8, as a key determinant protecting MYCN-amplified neuroblastoma cells from ferroptosis.

    Article Title: Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional programs.
    Article Snippet: Single guide RNA (sgRNA) sequences for non-targeting control (NTC) and ACE2 were taken from the Weissman Human Genome-wide CRISPRa-v2 library (Addgene #83978). .. LRRC15 sgRNA sequences and additional ACE2 sgRNA sequences were taken from the Human CRISPR activation pooled library set A (Addgene #92379). .. Sense and antisense strands for each sequence were ordered as DNA oligonucleotides (IDT) with 50 overhangs of 50-CACC-30 on the sense strand oligonucleotide and 50-AAAC-30 on the antisense strand oligonucleotide.

    Article Title: LRRC15 suppresses SARS-CoV-2 infection and controls collagen production
    Article Snippet: 375 Individual colonies were picked, expanded in Luria broth (Life Technologies) supplemented with 376 ampicillin and amplified constructs were harvested using either ISOLATE II Plasmid Mini Kit 377 (Bioline) or PureYield Plasmid Maxiprep System (Promega Corporation). .. 378 379 Whole genome sgRNA library amplification 380 MegaX DH10B T1R ElectrocompTM Cells (ThermoFisher Scientific) were electroporated with 400 381 ng Human CRISPR activation pooled library set A (Addgene #92379) and left to recover in 382 Recovery Medium for 1 hour at 37°C. .. Cells were then spread on 600 cm2 LB-agar plates 383 supplemented with carbenicillin (Merck) and incubated at 37°C for 16 hours.

    Activation Assay:

    Article Title: Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional programs
    Article Snippet: Single guide RNA (sgRNA) sequences for non-targeting control (NTC) and ACE2 were taken from the Weissman Human Genome-wide CRISPRa-v2 library (Addgene #83978). .. LRRC15 sgRNA sequences and additional ACE2 sgRNA sequences were taken from the Human CRISPR activation pooled library set A (Addgene #92379). .. Sense and antisense strands for each sequence were ordered as DNA oligonucleotides (IDT) with 5′ overhangs of 5′-CACC-3′ on the sense strand oligonucleotide and 5′-AAAC-3′ on the antisense strand oligonucleotide.

    Article Title: LRP8‐mediated selenocysteine uptake is a targetable vulnerability in MYCN‐amplified neuroblastoma
    Article Snippet: Following antibodies were used against β‐actin (1:5,000; A5441, Sigma‐Aldrich), GPX4 (1:1,000; no. ab125066, abcam), LRP8 (1:1,000; ab108208, abcam), SLC3A2 (CD98, 1:1,000; sc‐376815, Santa Cruz), SLC7A11 (1:10; antibody raised against a N‐terminal peptide of SLC7A11, clone 15C2‐111, developed in Munich + NaN 3 ), SCLY (1:50; IG‐P1097, ImmunoGlobe GmbH), Flag (1:3,000; F3165, Sigma‐Aldrich), GPX1 (1:1,000; #3286, Cell Signaling), mouse Slc7a11 (1:10; monoclonal antibody generated at the HMGU, clone 4B3‐1113, developed in Munich + NaN 3 ). .. The human CRISPR activation pooled library Set A (Addgene plasmid #92379 was a gift from David Root and John Doench) was amplified as described previously (Joung et al , ). .. Briefly, Endura electrocompetent cells (Lucigen, Cat. No. 60242) were used to perform six electroporation reactions on a MicroPulserTM II (BioRad) following the manufacturer's instructions (pre‐set EC1 setting with V: 1.8 kV).

    Article Title: Selenocysteine metabolism is a targetable vulnerability in MYCN -amplified cancers
    Article Snippet: Cell lines were tested for their identity by SNP genotyping and for mycoplasma contamination by the respective Multiplexion service (Heidelberg, Germany). .. The human CRISPR activation pooled library Set A (Addgene plasmid #92379 was a gift from David Root and John Doench) was amplified as described previously ( ). .. Briefly, Endura electrocompetent cells (Lucigen, Cat. No. 60242) were used to perform six electroporation reactions on a MicroPulserTM II (BioRad) following the manufacturer’s instructions (pre-set EC1 setting with V: 1.8 kV).

    Article Title: Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional programs.
    Article Snippet: .. MegaX DH10B T1R Electrocomp Cells (Thermo Fisher Scientific) were electroporated with 400 ng Human CRISPR activation pooled library set A (Addgene #92379) and left to recover in Recovery Medium for 1 h at 37 ̊C. .. Cells were then spread on 600 cm2 LB-agar plates supplemented with carbenicillin (Merck) and incubated at 37 ̊C for 16 h. All colonies were scraped, collected, and processed using the PureYield Plasmid Maxiprep System (Promega Corporation).

    Article Title: LRP8-mediated selenocysteine uptake is a targetable vulnerability in MYCN-amplified neuroblastoma.
    Article Snippet: Ferroptosis has emerged as an attractive strategy in cancer therapy.. Understanding the operational networks regulating ferroptosis may unravel vulnerabilities that could be harnessed for therapeutic benefit.. Using CRISPR-activation screens in ferroptosis hypersensitive cells, we identify the selenoprotein P (SELENOP) receptor, LRP8, as a key determinant protecting MYCN-amplified neuroblastoma cells from ferroptosis.

    Article Title: Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional programs.
    Article Snippet: Single guide RNA (sgRNA) sequences for non-targeting control (NTC) and ACE2 were taken from the Weissman Human Genome-wide CRISPRa-v2 library (Addgene #83978). .. LRRC15 sgRNA sequences and additional ACE2 sgRNA sequences were taken from the Human CRISPR activation pooled library set A (Addgene #92379). .. Sense and antisense strands for each sequence were ordered as DNA oligonucleotides (IDT) with 50 overhangs of 50-CACC-30 on the sense strand oligonucleotide and 50-AAAC-30 on the antisense strand oligonucleotide.

    Article Title: LRRC15 suppresses SARS-CoV-2 infection and controls collagen production
    Article Snippet: 375 Individual colonies were picked, expanded in Luria broth (Life Technologies) supplemented with 376 ampicillin and amplified constructs were harvested using either ISOLATE II Plasmid Mini Kit 377 (Bioline) or PureYield Plasmid Maxiprep System (Promega Corporation). .. 378 379 Whole genome sgRNA library amplification 380 MegaX DH10B T1R ElectrocompTM Cells (ThermoFisher Scientific) were electroporated with 400 381 ng Human CRISPR activation pooled library set A (Addgene #92379) and left to recover in 382 Recovery Medium for 1 hour at 37°C. .. Cells were then spread on 600 cm2 LB-agar plates 383 supplemented with carbenicillin (Merck) and incubated at 37°C for 16 hours.

    Plasmid Preparation:

    Article Title: LRP8‐mediated selenocysteine uptake is a targetable vulnerability in MYCN‐amplified neuroblastoma
    Article Snippet: Following antibodies were used against β‐actin (1:5,000; A5441, Sigma‐Aldrich), GPX4 (1:1,000; no. ab125066, abcam), LRP8 (1:1,000; ab108208, abcam), SLC3A2 (CD98, 1:1,000; sc‐376815, Santa Cruz), SLC7A11 (1:10; antibody raised against a N‐terminal peptide of SLC7A11, clone 15C2‐111, developed in Munich + NaN 3 ), SCLY (1:50; IG‐P1097, ImmunoGlobe GmbH), Flag (1:3,000; F3165, Sigma‐Aldrich), GPX1 (1:1,000; #3286, Cell Signaling), mouse Slc7a11 (1:10; monoclonal antibody generated at the HMGU, clone 4B3‐1113, developed in Munich + NaN 3 ). .. The human CRISPR activation pooled library Set A (Addgene plasmid #92379 was a gift from David Root and John Doench) was amplified as described previously (Joung et al , ). .. Briefly, Endura electrocompetent cells (Lucigen, Cat. No. 60242) were used to perform six electroporation reactions on a MicroPulserTM II (BioRad) following the manufacturer's instructions (pre‐set EC1 setting with V: 1.8 kV).

    Article Title: Selenocysteine metabolism is a targetable vulnerability in MYCN -amplified cancers
    Article Snippet: Cell lines were tested for their identity by SNP genotyping and for mycoplasma contamination by the respective Multiplexion service (Heidelberg, Germany). .. The human CRISPR activation pooled library Set A (Addgene plasmid #92379 was a gift from David Root and John Doench) was amplified as described previously ( ). .. Briefly, Endura electrocompetent cells (Lucigen, Cat. No. 60242) were used to perform six electroporation reactions on a MicroPulserTM II (BioRad) following the manufacturer’s instructions (pre-set EC1 setting with V: 1.8 kV).

    Article Title: LRP8-mediated selenocysteine uptake is a targetable vulnerability in MYCN-amplified neuroblastoma.
    Article Snippet: Ferroptosis has emerged as an attractive strategy in cancer therapy.. Understanding the operational networks regulating ferroptosis may unravel vulnerabilities that could be harnessed for therapeutic benefit.. Using CRISPR-activation screens in ferroptosis hypersensitive cells, we identify the selenoprotein P (SELENOP) receptor, LRP8, as a key determinant protecting MYCN-amplified neuroblastoma cells from ferroptosis.

    Amplification:

    Article Title: LRP8‐mediated selenocysteine uptake is a targetable vulnerability in MYCN‐amplified neuroblastoma
    Article Snippet: Following antibodies were used against β‐actin (1:5,000; A5441, Sigma‐Aldrich), GPX4 (1:1,000; no. ab125066, abcam), LRP8 (1:1,000; ab108208, abcam), SLC3A2 (CD98, 1:1,000; sc‐376815, Santa Cruz), SLC7A11 (1:10; antibody raised against a N‐terminal peptide of SLC7A11, clone 15C2‐111, developed in Munich + NaN 3 ), SCLY (1:50; IG‐P1097, ImmunoGlobe GmbH), Flag (1:3,000; F3165, Sigma‐Aldrich), GPX1 (1:1,000; #3286, Cell Signaling), mouse Slc7a11 (1:10; monoclonal antibody generated at the HMGU, clone 4B3‐1113, developed in Munich + NaN 3 ). .. The human CRISPR activation pooled library Set A (Addgene plasmid #92379 was a gift from David Root and John Doench) was amplified as described previously (Joung et al , ). .. Briefly, Endura electrocompetent cells (Lucigen, Cat. No. 60242) were used to perform six electroporation reactions on a MicroPulserTM II (BioRad) following the manufacturer's instructions (pre‐set EC1 setting with V: 1.8 kV).

    Article Title: Selenocysteine metabolism is a targetable vulnerability in MYCN -amplified cancers
    Article Snippet: Cell lines were tested for their identity by SNP genotyping and for mycoplasma contamination by the respective Multiplexion service (Heidelberg, Germany). .. The human CRISPR activation pooled library Set A (Addgene plasmid #92379 was a gift from David Root and John Doench) was amplified as described previously ( ). .. Briefly, Endura electrocompetent cells (Lucigen, Cat. No. 60242) were used to perform six electroporation reactions on a MicroPulserTM II (BioRad) following the manufacturer’s instructions (pre-set EC1 setting with V: 1.8 kV).

    Article Title: LRP8-mediated selenocysteine uptake is a targetable vulnerability in MYCN-amplified neuroblastoma.
    Article Snippet: Ferroptosis has emerged as an attractive strategy in cancer therapy.. Understanding the operational networks regulating ferroptosis may unravel vulnerabilities that could be harnessed for therapeutic benefit.. Using CRISPR-activation screens in ferroptosis hypersensitive cells, we identify the selenoprotein P (SELENOP) receptor, LRP8, as a key determinant protecting MYCN-amplified neuroblastoma cells from ferroptosis.

    Library Amplification:

    Article Title: LRRC15 suppresses SARS-CoV-2 infection and controls collagen production
    Article Snippet: 375 Individual colonies were picked, expanded in Luria broth (Life Technologies) supplemented with 376 ampicillin and amplified constructs were harvested using either ISOLATE II Plasmid Mini Kit 377 (Bioline) or PureYield Plasmid Maxiprep System (Promega Corporation). .. 378 379 Whole genome sgRNA library amplification 380 MegaX DH10B T1R ElectrocompTM Cells (ThermoFisher Scientific) were electroporated with 400 381 ng Human CRISPR activation pooled library set A (Addgene #92379) and left to recover in 382 Recovery Medium for 1 hour at 37°C. .. Cells were then spread on 600 cm2 LB-agar plates 383 supplemented with carbenicillin (Merck) and incubated at 37°C for 16 hours.



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    Image Search Results


    A) Overview of CRISPR screen methodology (adapted from ). B) SW620 KO screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. C) RKO KO screen;-log 10 RRA scores in cells treated with 1.9µM OxPt at Day 20 compared to control untreated cells at Day 20. D) SW620 CRISPRa screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. E) RKO CRISPRa screen;-log 10 RRA scores in cells treated with 1.9µM at Day 20 compared to control untreated cells at Day 20. In each graph, top 5 hits are labeled; significant hits are colored (blue or lilac) while non-significant hits are in grey.

    Journal: bioRxiv

    Article Title: A whole genomic CRISPR-Cas9 screen identifies the amino acid transporter SLC43A1 (LAT3) as a major determinant of oxaliplatin sensitivity in colorectal cancer cells

    doi: 10.1101/2025.04.21.649594

    Figure Lengend Snippet: A) Overview of CRISPR screen methodology (adapted from ). B) SW620 KO screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. C) RKO KO screen;-log 10 RRA scores in cells treated with 1.9µM OxPt at Day 20 compared to control untreated cells at Day 20. D) SW620 CRISPRa screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. E) RKO CRISPRa screen;-log 10 RRA scores in cells treated with 1.9µM at Day 20 compared to control untreated cells at Day 20. In each graph, top 5 hits are labeled; significant hits are colored (blue or lilac) while non-significant hits are in grey.

    Article Snippet: The Human Calabrese CRISPR Activation Pooled Library Set A was a gift from David Root and John Doench (Addgene viral prep #92379-LVC) ( ).

    Techniques: CRISPR, Control, Labeling

    LB-100 activates oncogenic signaling, engages stress response pathways, and restrains the proliferation of colorectal cancer cells. A , Gene set enrichment analyses on time-course transcriptome data from HT-29 and SW-480 cells show selected “Hallmarks” and “KEGG” molecular signatures modulated by LB-100 (4 μmol/L). Darker bars indicate time points for which the respective gene set was significantly enriched (P <0.05). B , Time-course western blots show selected oncogenic signaling and stress response pathways modulated by LB-100 (4 μmol/L) in HT-29 and SW-480 cells. α-Tubulin and Vinculin were used as loading controls. C , IncuCyte-based proliferation assays with the colorectal cancer models in the absence or presence of LB-100 at 1, 2, or 4 μmol/L for the indicated times. D , String network combining all hits identified by the two independent genome-wide CRISPR screens (Supplementary Fig. S2) as modulators of LB-100 toxicity. Only high-confidence interactions are shown and disconnected nodes are omitted. Green nodes: CRISPRa screen; orange nodes: CRISPR-KO screen; yellow node: identified on both screens. E , GO analyses using the full list of hits from both CRISPR screens (Supplementary Fig. S2) as input. The top 5 enriched Biological Processes and Molecular Functions terms are shown. Darker bars highlight WNT/β-catenin-and MAPK-related terms.

    Journal: Cancer Discovery

    Article Title: Paradoxical Activation of Oncogenic Signaling as a Cancer Treatment Strategy

    doi: 10.1158/2159-8290.CD-23-0216

    Figure Lengend Snippet: LB-100 activates oncogenic signaling, engages stress response pathways, and restrains the proliferation of colorectal cancer cells. A , Gene set enrichment analyses on time-course transcriptome data from HT-29 and SW-480 cells show selected “Hallmarks” and “KEGG” molecular signatures modulated by LB-100 (4 μmol/L). Darker bars indicate time points for which the respective gene set was significantly enriched (P <0.05). B , Time-course western blots show selected oncogenic signaling and stress response pathways modulated by LB-100 (4 μmol/L) in HT-29 and SW-480 cells. α-Tubulin and Vinculin were used as loading controls. C , IncuCyte-based proliferation assays with the colorectal cancer models in the absence or presence of LB-100 at 1, 2, or 4 μmol/L for the indicated times. D , String network combining all hits identified by the two independent genome-wide CRISPR screens (Supplementary Fig. S2) as modulators of LB-100 toxicity. Only high-confidence interactions are shown and disconnected nodes are omitted. Green nodes: CRISPRa screen; orange nodes: CRISPR-KO screen; yellow node: identified on both screens. E , GO analyses using the full list of hits from both CRISPR screens (Supplementary Fig. S2) as input. The top 5 enriched Biological Processes and Molecular Functions terms are shown. Darker bars highlight WNT/β-catenin-and MAPK-related terms.

    Article Snippet: HT29 dCas9-VP64 clone E cells were transduced with lentivirus of Calabrese pooled human CRISPRa library sets A and B (Addgene, 92379 and 92380) separately, in the presence of 8 μg/mL polybrene (Santa Cruz, sc-134220A), and at an MOI of approximately 0.3.

    Techniques: Western Blot, Genome Wide, CRISPR